Effect of zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by L-NAME.

Corresponding author: OU Hesheng., hs_ou01@163.com

DOI: 10.12201/bmr.202503.00028

•  

  Abstract: Objective To investigate the effect of zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by L-NAME and its possible molecular mechanism. Methods L-NAME was used to induce a hypertension rat model. According to the experimental group, they were divided into blank group, model group, Captopril group, and high, medium, and low dose groups of Shiya tea.and the experimental groups were divided into blank group, model group, Captopril group, and high, medium, and low dose groups of Adinandra nitida. The rats were continuous gavaged for 4 weeks, and the blood pressures were measured once a week. The last blood pressure was measured 1 hour after the last administration. Rats were anesthetized by intraperitoneal injection, tissues and blood samples were collected. Pathological changes of the rat thoracic aortic tissue were observed by hematoxylin Eosin (HE) staining, the protein expression level of endothelial nitric oxide synthase (eNOS) in rat thoracic aorta was detected by western blotting, the content of eNOS in rat plasma was measured by enzyme linked immunosorbent assay (ELISA) ,and the nitric oxide (NO) content in rat plasma was detected by One step reduction method. Results Compared with the model group, the high and medium dose groups of Adinandra nitida and the Captopril group of rats showed significant reductions in systolic blood pressure and diastolic blood pressure (P< 0.05 or P< 0.01), while there was no statistically significant change in heart rate (P>0.05). After 4 weeks of oral administration with Adinandra nitida, the damage of the vascular tissues of hypertensive rats induced by L-NAME has been improved, the expression of eNOS protein in thoracic aortic blood was significantly increased (P<0.01), and the contents of eNOS and NO in rat plasma were significantly increased (P<0.01). Conclusion Zhuang medicine Adinandra nitida has a hypotensive effect on L-NAME induced hypertensive rats, which may be related to the regulation of eNOS expression.

  Key words: Adinandra nitida; L-NAME; Hypertension; Endothelial nitric oxide synthase; Nitric oxide

  Submit time: 11 March 2025

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
• html

• 图表

• Liu Jianyi, Liu Yan, Gao Yunchun, Jin Ling. Risk factors and prognostic analysis of early myocardial injury after acute carbon monoxide poisoning. 2025. doi: 10.12201/bmr.202504.00060

  ZHANG Xiao-juan. Study on the Prevention and Treatment of Hypertension and Diabetes Mellitus in Community Hospital. 2023. doi: 10.12201/bmr.202311.00002

  YUE xiaobo, ZHAO Xingsheng. The correlation between serum MHR, NLR, and PLR with essential hypertension. 2024. doi: 10.12201/bmr.202411.00052

  zhouyibo, wangxidan, chenjingwan, chenfuhua, taoxiaoying. YANG Daoling, ZHOU Yibo, WANG Xidan, CHEN Jingwan, CHEN Fuhua, TAO XiaoyingFirst-author’s address: Department of Ultrasound Medicine, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China. 2024. doi: 10.12201/bmr.202410.00076

  LI Chao. Study on the diagnostic value of serum miR-181b and lncRNA MIAT in hypertensive nephropathy. 2025. doi: 10.12201/bmr.202504.00051

  XU Jianhong. Study of dexmedetomidine on intestinal barrier protection in hypertensive patients. 2024. doi: 10.12201/bmr.202408.00045

  Yaoqian. The effect of polydatin on myocardial injury in rats with doxorubicin induced dilated cardiomyopathy by regulating the Shh/Gli1 signaling pathway. 2025. doi: 10.12201/bmr.202506.00013

  pangzhen, GuJiYu, WuYuFei, YanSshiXing, LiWangYang, SunYue. A study on the solution of the problem of extracting essential substance of TCM diagnosis and treatment of hypertension based on triple extraction strategy. 2021. doi: 10.12201/bmr.202107.00015

  zhou wei. Construction and Analysis of a Prediction Model for Hypertension Combined with Left Ventricular Diastolic Dysfunction Based on Random Forest AlgorithmWANG Tingting1 ,ZHOU Wei1*. 2025. doi: 10.12201/bmr.202503.00046

  Correlation analysis betweensevere acute respiratory syndrome coronavirus 2 infection and Henoch Sch?nlein purpura nephritis. 2024. doi: 10.12201/bmr.202409.00034

• ID	Submit time	Number	Download
  1	2025-02-15	bmr.202503.00028V1	Download

• Public  Anonymous  To author only Submit Comments