CHEN Li,ZHAO Ming-Zhe*,ZHU Ting-Jun,XIA Bing－Tian,LI Lu－Lu

Corresponding author: ZHAO Ming-Zhe, doctorzmz@126.com

DOI: 10.12201/bmr.202502.00041

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  Abstract: Objective：This study aims to examine the elements influencing the mobilization and retrieval of hematopoietic stem cells from peripheral blood Objective：: This study aims to examine the factors that affect the mobilization and collection of peripheral blood hematopoietic stem cells in patients diagnosed with malignant hematological disorders. Additionally, it seeks to investigate the predictive significance of platelet levels during the autologous stem cell collection process. Methods：Clinical data of 52 cases of patients with malignant diseases of the haematological system who underwent autologous stem cell mobilisation and collection from September 2018 to March 2024 in the Department of Haematology of Jinhua Central Hospital were retrospectively analysed. Factors such as patients age, gender, number of chemotherapy cycles, mobilisation regimen, collection machine, and whether the bone marrow was in remission were analysed to assess the influence of the above factors on CD34+ counts in stem cell collection, and the correlation between the number of leukocytes, monocytes, lymphocytes, and platelets prior to collection and high-quality mobilisation was also analysed. The optimal cut-off value of platelets before collection was calculated by applying the receiver operator characteristic curve (ROC curve).Results: Effectiveness of haematopoietic stem cell mobilisation and collection Fifty-two patients underwent peripheral blood haematopoietic stem cell collection, and the median number of CD34+ cells obtained was 6.935 (4.046-10.37) × 106/kg. The quality of mobilisation was high in 36 cases (high quality mobilisation group) and average in 16 cases (non-high quality mobilisation group). The number of pre-collection chemotherapy cycles and mobilisation regimen had a significant effect on the number of CD34+ cells collected, whereas age, gender, collection machine, and bone marrow involvement or not had no significant effect on the number of stem cells collected. Univariate analysis of platelet count was associated with quality mobilisation (P＜0.05), whereas but the difference was not statistically significant when comparing WBC, MNC and ALC before collection, (P＞0.05). Further multifactorial analysis showed that the shadow of platelets on quality collection was statistically significant (OR=0.975, 95% CI: 0.954-0.997).Pre-collection platelet count correlated with quality collection and the optimal cut-off cut-off value for pre-collection PLT was 0.535. Conclusion: Multi-course chemomerapy before collection is poor factor negatively impacting on auto-PBSCs mobilization．Non-steady-state mobilisation chemotherapy regimens collected a better number of CD34+ cells than steady state mobilisation (plerixafor) regimens.Determining the appropriate timing of collection by pre-collection platelet count can improve the success of collection. Platelets are not only associated with quality mobilization, but may also be an important factor in promoting stem cell production.

  Key words: malignant hematological disease; autologous peripheral blood stem cell transplantation; mobilization; PLT

  Submit time: 21 February 2025

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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