通讯作者: 潘婧婧, 330740365@qq.com

DOI：10.12201/bmr.202505.00011

Analysis of Occult Hepatitis B Virus Infection Status among Blood Donors in Wenzhou Area

• 摘要：目的 　探讨温州地区无偿献血人群隐匿性乙型肝炎病毒感染状况。方法 选取温州地区2022年12月-2024年4月自愿参与无偿献血人员年龄为18-60周岁的血液158256份进行乙型肝炎表面抗原(HBsAg)血清学检测和HBV-DAN核酸检测,并对HBsAg(-)/HBV-DNA(+)样本进行乙肝血清学标志物检测和HBV-DNA病毒载量测定,并对检测结果进行统计分析。结果 158256例献血者,共检出OBI献血者148例,OBI检出率为0.094％。不同年龄段献血者人群中,41~55岁OBI检出率最高,差异均有统计学意义(X2=93.986,P＜0.05)。OBI血清学模式共6种,其中抗HBs和抗HBc联合阳性占比最高(36.47％,54/148),单独抗-HBc阳性次之(20.27％,30/148)；比较OBI献血者的抗-HBS、抗-Hbe、抗-HBc阳性率,有统计学意义,以抗-HBc阳性率最高,达90.54％,抗-HBs阳性率次之,为62.84％(X2=97.28,P＜0.05)。148例OBI标本中,32例检测到病毒载量,检出率为21.62％,HBV-DNA病毒载量范围为1.39~98.82IU/ml(中位数8.34 IU/ml),以抗-HBc(+)/抗-HBe(+)为主。比较抗-HBs阳性病毒载量检出率与阴性组检出率,有统计学差异(X2=17.45,P＜0.05)。结论 温州地区存在一定的OBI感染率,核酸检测并行ELISA检测有效降低经血传播HBV风险。年轻的献血者和56周岁以上固定献血者OBI感染率较低,加强固定献血者队伍建设,进一步提高血液的安全性。

  关键词： 献血者; 温州地区; 隐匿性乙型肝炎病毒感染; 抗-HBc; 核酸检测

   

  Abstract: Objective To investigate the status of occult hepatitis B virus (HBV) infection among voluntary blood donors in Wenzhou. Methods A total of 158,256 blood samples from voluntary blood donors aged 18 to 60 years in Wenzhou from December 2022 to April 2024 were tested for hepatitis B surface antigen (HBsAg) serology and HBV-DNA nucleic acid. HBsAg(-)/HBV-DNA(+) samples were further tested for hepatitis B serological markers and HBV-DNA viral load. The test results were statistically analyzed. Results Among 158,256 blood donors, 148 cases of OBI were detected, with an OBI detection rate of 0.094%. Among different age groups of blood donors, the OBI detection rate was the highest in the 41-55 age group, with statistically significant differences (X2 = 93.986, P < 0.05). There were six serological patterns of OBI, among which the combined positive rate of anti-HBs and anti-HBc was the highest (36.47%, 54/148), followed by the positive rate of anti-HBc alone (20.27%, 30/148). The positive rates of anti-HBs, anti-HBe, and anti-HBc in OBI donors were statistically significant, with the highest positive rate of anti-HBc at 90.54%, followed by anti-HBs at 62.84% (X2 = 97.28, P < 0.05). Among the 148 OBI specimens, 32 cases had detectable viral loads, with a detection rate of 21.62%. The HBV-DNA viral load ranged from 1.39 to 98.82 IU/ml (median 8.34 IU/ml), mainly in the anti-HBc(+)/anti-HBe(+) pattern. There was a statistically significant difference in the detection rate of viral load between the anti-HBs positive group and the negative group (X2 = 17.45, P < 0.05). Conclusion There is a certain rate of OBI infection in Wenzhou. The combination of nucleic acid testing and ELISA testing effectively reduces the risk of HBV transmission through blood. Young blood donors and regular blood donors over 56 years old have a relatively low OBI infection rate. Strengthening the construction of the regular blood donor team can further improve the safety of blood.

  Key words: Blood donors; Wenzhou; Occult hepatitis B virus infection; Anti-HBc nucleic acid testing (NAT); 

  提交时间：2025-05-13

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• 序号	提交日期	编号	操作
  1	2025-04-10	bmr.202505.00011V1	下载

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