通讯作者: 安淑红, 68279553@qq.com

DOI：10.12201/bmr.202403.00019

Expression and the role of TFF3 in lung adenocarcinoma based on bioinformatics analysis

• 摘要：摘 要： [目的] 采用生物信息学方法和体外细胞实验探索TFF3在肺腺癌中的作用。 [方法] 基于TIMER、UALCAN数据库分析TFF3在癌组织中的表达。基于TCGA数据库和R包，从临床特征、免疫微环境、肿瘤突变负荷、免疫治疗等多个角度分析TFF3高低表达患者组的特征差异。通过RTCA检测TFF3对NCI-H1975细胞数目的影响，通过Western blot检测PI3K/Akt信号通路Akt及其激活状态p-Akt的表达。 [结果] TFF3在肺腺癌组织中表达增高，表达量与肺腺癌的病理类型有关，但与患者预后无关。TFF3低表达与低肿瘤纯度相关。TFF3和肿瘤突变负荷、数种免疫检查点基因表达呈负相关。TFF3处理NCI-H1975细胞后，细胞总数上升。Western blot结果表明，TFF3能激活p-Akt基因表达。 [结论] TFF3在某些肺腺癌亚型中表达升高，且与肺腺癌肿瘤免疫具有一定关联。TFF3可促进肺腺癌细胞增殖，并可能通过激活PI3K/Akt信号通路发挥作用。TFF3可能成为肺腺癌患者一个新的治疗靶点

  关键词： 关键词：TFF3 肺腺癌 生物信息学分析 肿瘤免疫 PI3K/Akt

   

  Abstract: Abstract: Objective Exploring the role of TFF3 in lung adenocarcinoma using bioinformatics methods and in vitro cell experiments. Methods This study aims to analyze the characteristic differences between patient groups with high and low TFF3 expression using the TCGA database and R package. The analysis will cover various perspectives, including clinical features, immune microenvironment, tumor mutation burden, and immunotherapy. The effect of TFF3 on the number of NCI-H1975 cells was determined using RTCA, while the expression of the PI3K/Akt signaling pathway Akt and its activated state p-Akt was detected using Western blot. Results The expression of TFF3 was found to be increased in lung adenocarcinoma tissue, and this increase was found to be related to the pathological type of lung adenocarcinoma. However, it was not found to be associated with the prognosis of patients. Low expression of TFF3 was found to be associated with low tumor purity. Additionally, TFF3 was found to have a negative correlation with tumor mutation burden and the expression of several immune checkpoint genes. In our study, we treated NCI-H1975 cells with TFF3 and observed an increase in the total number of cells. Furthermore, the results from Western blot analysis indicated that TFF3 can activate the expression of the p-Akt gene. Conclusions TFF3 is found to be upregulated in specific subtypes of lung adenocarcinoma and is closely linked with tumor immunity in this type of cancer. It has been observed that TFF3 can enhance the growth of lung adenocarcinoma cells, possibly by activating the PI3K/Akt signaling pathway. These findings suggest that TFF3 could potentially serve as a promising therapeutic target for individuals diagnosed with lung adenocarcinoma.

  Key words: TFF3 Lung adenocarcinoma Bioinformatics analysis PI3K/Akt

  提交时间：2024-04-15

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• 序号	提交日期	编号	操作
  1	2024-03-29	bmr.202403.00019V1	下载

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